Category: Sepsis

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Conclusion

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Conclusion

To further support our suggestion that dobutamine may have functionally reduced the effects of altered endothelial permeability characteristics on microvascular fluid flux in this study, Hakim et al concluded that (3-adrenergic receptors regulated pulmonary transendothelial transport of fluid and proteins since (3-adrenergic blockade with propranolol was associated with an increase in QL. Also, in the…

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Observations

Figure 1. Changes in cardiac output, lung lymph flow, [L/P]TP ratios, and mean pulmonary arterial wedge pressure during one experiment.

Nonseptic Study The infusion of dobutamine was associated with a modest fall in the mean blood pressure at the 10μg/kg/ min dose but was without effect on the mean pulmonary arterial pressure at either dose (Table 1 ana r lg l). The cardiac output increased progressively with both doses, while the measured pulmonary arterial wedge…

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Specific Measurements

Specific Measurements We collected pulmonary lymph in graduated heparinized tubes. From pooled lymph specimens, we measured total protein and albumin concentrations (in grams per deciliter) by the Biuret method using an automated system (Auto Analyzer, Technicon Instruments); duplicate samples differed by less than 5 percent. At the time of lymph collection, we also measured total…

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Experimental Protocol

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Experimental Protocol

Experimental Protocol Baseline studies were performed over a 120-minute period approximately three to four days after recovery from preliminary surgery. Pulmonary lymph was collected and measured for volume every 15 minutes; lymph was pooled at the end of the two-hour baseline period for measurement of total protein and albumin. At the midpoint of this baseline…

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Materials and Methods

Experimental Preparation Ten mature Suffolk sheep, weighing 30 to 40 kg (0.9 to 1.2 m2 of body surface area), were prepared with chronic lymph fistulae using a modification of the technique described by Staub et al. Before study the sheep were premedicated with atropine sulfate and were then anesthetized and intubated. We cannulated the aorta…

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Outcome

Dobutamine had no net effect on pulmonary fluid flux in the nonseptic studies. In contrast, its effects in the septic state were inconsistent. At a dose of 5μg/kg/ min, a modest (24 percent) increase in QL was demonstrable when compared to baseline, while no significant change was evident with the 10μLg/kg/min dose; with an increase…

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Lungs microvascular

Effect of Dobutamine on Lung Microvascular Fluid Flux in Sheep with “Sepsis Syndrome”: Lungs microvascular

Since dobutamine has been recommended as an adrenergic receptor agonist which might be used in preference to dopamine when systemic flows require pharmacologic support in ARDS, we studied its effects on fluid flux in an animal model of peritonitis which is characterized by lung microvascular injury. This model seems more representative of early clinical sepsis…

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